If you did not compensate on the machine or use fcs2.0 data, the parameters will not have the comp-prefix.
#Flowjo 10 apply compensation matrix software#
We’re here to help you accelerate routine phenotyping, take your immunology research to the next level, and get you from data to results―one cell at a time.įlowJo applies the compensation matrix generated by acquisition software automatically upon loading data. In spectral flow, light is collected in all detectors for allįlowJo is your biggest fan and strives to be an outstanding source of support. Also, there may be casesĪccelerate your discovery with the latest features that come with FlowJo v10.6.1: Spectral compensation allows users who collect more channels than they have parameters to get cleaner data if properly done. This may be necessary in cases where the compensation was inappropriately set during sample collection (although if the sample was over-compensated, then there is no recourse). In this video, FlowJo Application Scientist Mike Stadnisky introduces you to the newest version of FlowJo with a 24-part run throuįlowJo gives you the ability to compensate your data. The detectors, or channels, in the instrument are designed to detect a very specific range of Compensation controls (single stain samples) must now belong to a compensation group inĬompensation has undergone a major redesign for FlowJo Version X! Compensation in flow cytometry is the process of correcting for fluorescence spillover emissions.
CHAPTER 2 – Creating a FlowJo compensation matrix from single stain controls back to Compensation Workflow Overview Compensation has been reworked for version 10.
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